Alpha-amylase, an enzyme involved in breaking down carbohydrates, increases in response to stress as part of the body's "fight or flight" activation via the sympathetic nervous system. Elevated alpha-amylase levels indicate heightened physiological arousal and are linked to acute stress responses, such as those triggered by psychological or physical challenges. Chronic elevation of this biomarker has been associated with increased risks of cardiovascular issues, anxiety, and impaired cognitive function. In the context of neurological health, alpha-amylase levels offer insights into how the body manages stress over time and how stress-related activation affects brain function and emotional regulation.
Alpha-amylase is measured non-invasively using saliva samples collected at specific times of the day, as the enzyme follows a diurnal pattern similar to cortisol. To obtain accurate results, individuals should avoid eating, drinking, smoking, or brushing their teeth at least 30 minutes before sample collection. Samples are typically analyzed in a laboratory using spectrophotometric or immunoassay techniques to quantify enzyme activity. Measuring alpha-amylase at multiple intervals, such as before and after a stress-inducing task, helps assess the body's acute stress response and recovery, providing a dynamic picture of stress regulation.